Green fluorescent protein expression triggers proteome changes in breast cancer cells

Title: Green fluorescent protein expression triggers proteome changes in breast cancer cells
Creator: Coumans, J. V. F.; Gau, D.; Poljak, A.; Wasinger, V.; Roy, P.; Moens, P.
Relation: Experimental Cell Research Vol. 320, Issue 1, p. 33-45
Publisher Link: http://dx.doi.org/10.1016/j.yexcr.2013.07.019
Publisher: Academic Press
Resource Type: journal article
Date: 2014
Description: Green fluorescent protein (GFP) is the most commonly used reporter of expression in cell biology despite evidence that it affects the cell physiology. The molecular mechanism of GFP-associated modifications has been largely unexplored. In this paper we investigated the proteome modifications following stable expression of GFP in breast cancer cells (MDA-MB-231). A combination of three different proteome analysis methods (2-DE, iTRAQ, label-free) was used to maximise proteome coverage. We found that GFP expression induces changes in expression of proteins that are associated with protein folding, cytoskeletal organisation and cellular immune response. In view of these findings, the use of GFP as a cell reporter should be carefully monitored.
Subject: GFP expression; proteomic change; unfolded protein response; cytoskeleton modifications; cellular immune response
Identifier: http://hdl.handle.net/1959.13/1304502
Identifier: uon:20859
Identifier: ISSN:0014-4827
Language: eng
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